Modulation of Radiation Induced Biochemical Alterations in Swiss Albino Mice by Grewia Asiatica (phalsa)

Increasing use of nuclear radiation for human welfare necessitates a new, safe and cost effective radio protector not only for personnel’s charged with responsibility of testing or with radiations in laboratories, but also for the general public. Keeping this view, this study has been undertaken to find out the possible radio protective potential of the Grewia asiatica fruit pulp extract (GAE), Grewia asiatica has a high content of antioxidants like Vitamin C, anthocyanin and folate that may play a possible role in radioprotection .For experimental study, healthy Swiss Albino mice were selected from an inbred colony and divided into four groups. Group I (normal) did not receive any treatment. Group II was orally supplemented (GAE) once daily at the dose of 700 mg / Kg. b.wt / day for fifteen consecutive days. Group III (control) received distilled water orally equivalent to GAE for fifteen days than exposed to 5 Gy of gamma radiation. Group IV (experimental) was administered orally (GAE) for 15 consecutive days once daily and exposed to single dose of 5Gy of gamma radiation. Mice were sacrificed at different autopsy intervals viz. 1, 3, 7, 15 and 30 days and blood and liver were removed for various biochemical estimations viz. glutathione (GSH), lipid peroxidation (LPO). Irradiation resulted an elevation in lipid peroxidation (LPO) and a decline in glutathione (GSH) level in liver as well as blood. On the other hand, treatment of animals with GAE extract before irradiation caused a significant decrease in LPO and a marked elevation in GSH.